The UBA domain

A sequence motif found in multiple enzyme classes of the ubiquitination pathway

This page is based on the publication:
Hofmann K. and Bucher P. (1996), The UBA-domain: A sequence motif found in multiple enzyme classes of the ubiquitination pathway Trends Biochem. Sci. 21 172-173.

and on the poster:
Hofmann K. and Bucher P. Conserved sequence domains in cell cycle regulatory proteins presented at the joint ISREC/AACR meeting "Cancer and the Cell cycle", January 1996 in Lausanne.

Short description

The UBA-domain is a novel sequence motif found in several proteins having connections to ubiquitin and the ubiquitination pathway:

Bovine E2-25K and several other ubiquitin-conjugating enzymes (E2), catalyzing the second step in protein ubiquitination.
Drosophila hyperplastic discs protein, a putative ubiquitin-protein ligase (E3), catalyzing the third and final step in protein ubiquitination.
Human ubiquitin isopeptidase T and several other ubiquitin C-terminal hydrolases, catalyzing regulatory protein-deubiquitination.
S.cerevisiae RAD23 and its mammalian homologues. These proteins act in UV excision repair and contain a N-terminal domain similar to ubiquitin itself.
Mammalian proto-oncogene c-cbl, a protein interacting with multiple signal transduction factors. Cbl has recently been shown to undergo regulatory ubiquination upon macrophage stimulation.

Also available is a more detailed description of the UBA domain and the relevant protein families.

PROSITE

The UBA domain will be included in PROSITE under the accession numbers PS50030 for the data entry, and PDOC50030 for the documentation entry. The alignment used for construction of the profile is available in MSF format. There is also an updated list of database sequences containing UBA domains.

Domain cartoons

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Abbreviations: U: UBA-domain; H1,H2: Deubiquitinase catalytic domains; UBC: UBC catalytic domain; ub: ubiquitin-homology; RF: RING-finger; pab: PABP C-terminus homology; KA1: KA1-domain; EH: EPS15-homology domain; HECT: Ubiquitin-ligase (E3) catalytic domain; UX: UX-domain.
Links to WWW-pages describing some of these domains can be found near the bottom of this page.

Alignment of representative sequences

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Note: You can check your sequence for the occurence of this and other domains by using the ProfileScan server.

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